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KMID : 0942820070060020110
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Journal of Korean Brain Tumor Society
2007 Volume.6 No. 2 p.110 ~ p.115
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Roles for tumor suppressor gene methylation on the meningioma grades and outcome
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Cho Ji-Young
Kim Jeong-Hoon
Hong Seock-Ho
Kim Chang-Jin
Lee Jung-Kyo
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Abstract
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Objective：Aberrant methylation of CpG islands in promoter of human genes is known as an alternative mechanism of gene silencing that contributes to tumorigenesis in various human tumors. DNA methylation status of tumor suppressor genes (TSGs) is poorly understood in meningiomas, so we performed this study to determine the roles for DNA methylation on the meningioma grades and outcome.
Methods： We have examined the methylation status of 5 TSGs in 81 human meningiomas(59 Grade I, 15 Grade II, and 7 Grade III) by methylation specific polymerase chain reaction(PCR).
Results： Five TSGs showed different profile of methylation status：46.9% for p16, 21.0% for p14, 42.0% for RB1, 7.4% for RASSF1A, and 42.0% for E-cadherin. DNA hypermethylation frequencies in p16, p14, RASSF1A, and E-cadherin were higher in Grade II than in Grade I and Grade III except RB1. There were no correlations of the methylation status of these TSGs with meningioma grades. Also, the methylation status of all TSGs was not associated with recurrence and survival.
Conclusion： Our results suggest that DNA methylation may be one of the important events in tumorigenesis of meningiomas and it may be a major molecular mechanism especially for Grade II meningiomas. The methylation status of TSGs is not useful to discriminate among histologic grades and outcome of meningiomas. Further molecular studies will be needed to predict the malignancy and biological behavior of meningiomas.
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KEYWORD
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Methylation, Tumor suppressor genes, Meningiomas, Grades, Outcome
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